- Protein crystallography
Main steps: - Protein purification - Crystallisation
Special: - Programs for crystallography - X-ray detectors
Basic tutorials: - Chemistry - Protein - Peptide - Amino Acids
Xtal community: - CCP4BB
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Subject: Re: How to subtract one electron density map from another From: Paul Emsley emsley {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK Date: 2007-03-22
On Thu, 2007-03-22 at 16:06 -0500, Qing Xie wrote: > I'm trying to get the difference map by subtracting the native electron > density map from the complex electron density map. MAPMASK has a function > of ADD/MULT, but I don't know how to use it? > Any other ways to attack this problem in real space?
use overlapmap with ADD 1 -1
presuming your maps are on the correct scale (if not you can use mapmask to scale (one of) them).
Paul.
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