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Re: [ccp4bb] crystallisation robot - volume? |
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CCP4bb navigationCCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008Subject: Re: crystallisation robot - volume? From: cdekker carien {- dot -} dekker {- at -} ICR {- dot -} AC {- dot -} UK Date: 2008-01-16 one would like to have protein in the right nucleation zone for a certain period of time. If the drop becomes too small, the ratio evaporation surface/drop volume changes. So, the nucleation zone might be reached within the drop but either too fast ('racing through the phase diagram too quickly') or in the smaller droplet turbulence caused by evaporation might be more pronounced and interfere with nucleation. At some stage I looked at this myself, although not for tiny volumes (0.3, 0.5 and 0.8ul droplets). With smaller volume I saw more and faster nucleation, but less crystal growth. There's got to be an optimum in terms of drop volume when using vapour diffusion. Any comments? Carien On 16 Jan 2008, at 13:17, Anastassis Perrakis wrote: >> Oryxnano 50+50 nL >> >> Demetres >> > > Which, indirectly, brings up an interesting (but not relevant to the > Oryx) question. > > Nucleation is a process that does have a stochastic aspect. > > Thus, one could argue that compromising to 200-300 nl might be better > than either extremes of 50nl (too small volume and less chance for > nucleation) or 1000 nl (too much sample). > > any comments ? (let the flames begin). > > A. > > PS1 > another interesting issue that has has been hardly touched in these > emails is the real sample loss: left in wells and not easy to recover, > lost because of contamination with system liquid, etc ... > > PS2 > I see lots of people with new robots. please do have a look at the > www.BIOXHIT.org page and if you have a few minutes to assemble a table > we will be happy to add your specs to our pages. it can be a nice > resource and it has already enough things and already one response to > my last email ;-) To make life easier to potential contributors we can > provide an Excel sheet to fill up with your specs - just ask. > > On Jan 16, 2008, at 12:46, Demetres D. Leonidas wrote: > >> >> David Briggs wrote: >>> I'll defend the honour of the phoenix... (again) >>> >>> Bernhard Rupp 100+100 nl >>> Dave Briggs (and all users at Univ of Manchester, UK) 100+100nl >>> Others.. >>> >>> Only time we have ANY problems is when the nano dispensing tip gets >>> clogged. Often a good wash whilst still on the machine will clear >>> the blockage. >>> >>> Dave >>> >>> >>> >>> >>> -- >>> ============================ >>> David C. Briggs PhD >>> Father & Crystallographer >>> http://www.dbriggs.talktalk.net >>> AIM ID: dbassophile >>> ============================ >> >> -- >> Demetres D. Leonidas, Ph.D. >> Structural Biology & Chemistry Group >> Institute of Organic and Pharmaceutical Chemistry >> The National Hellenic Research Foundation >> 48, Vassileos Constantinou Avenue >> Athens 116 35, Greece >> ================================================== >> Tel. +30 210 7273841 (office) >> +30 210 7273895 (lab) Fax. +30 210 7273831 >> E-mail: ddl@eie.gr >> URL: http://athena.eie.gr >> ================================================== The Institute of Cancer Research: Royal Cancer Hospital, a charitable Company Limited by Guarantee, Registered in England under Company No. 534147 with its Registered Office at 123 Old Brompton Road, London SW7 3RP. This e-mail message is confidential and for use by the addressee only. If the message is received by anyone other than the addressee, please return the message to the sender by replying to it and then delete the message from your computer and network. CCP4bb navigationCCP4bb <-- 2008 <-- January 2008 <-- 16 January 2008 |
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