[ccp4bb] Inclusion body

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Nature policy update regarding source code
From: "Nadir T {- dot -} Mrabet" Nadir {- dot -} Mrabet {- at -} MEDECINE {- dot -} UHP-NANCY {- dot -} FR
Date: 2007-03-23

Next message:
Subject: Re: Inclusion body
From: verdino {- at -} SCRIPPS {- dot -} EDU verdino {- at -} SCRIPPS {- dot -} EDU
Date: 2007-03-23

Subject: Inclusion body
From: Weijun Huang weijun {- at -} KU {- dot -} EDU
Date: 2007-03-23

Dear All,

I got enclusion body in many cases when I tried to express human
proteins in E coli. I would like some suggestions on how to go about
it. I would also like to try co-expression of GroEL/GroES or
DnaJ/DnaK, and would like to know where to get the plasmids.

Any help or comments would be appreciated.
Weijun

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: Inclusion body
From: verdino {- at -} SCRIPPS {- dot -} EDU verdino {- at -} SCRIPPS {- dot -} EDU
Date: 2007-03-23