Re: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

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CCP4bb <-- 2008 <-- January 2008 <-- 18 January 2008

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Subject: Re: differences between Rsym and Rmerge
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2008-01-18

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Subject: Re: differences between Rsym and Rmerge
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-01-18

Subject: Re: Fwd: crystallisation robot
From: Lisa A Nagy lisanagy {- at -} UAB {- dot -} EDU
Date: 2008-01-18

Al's Oil on the plates:
What a nightmare!!!!!!!
The oil creeps up the plate and over the sides. It dissolves adhesives.
It makes me say bad words in multiple languages.
Bigger drops + no oil = fewer bad words.

Lisa
--
Lisa A. Nagy, Ph.D.
University of Alabama-Birmingham
nagy@uab.edu

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Patrick Shaw Stewart
Sent: Friday, January 18, 2008 2:20 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Fwd: [ccp4bb] crystallisation robot

One thing that people often overlook is that quite a lot of protein
can be lost by denaturation on the surface of the drop. This is more
significant for smaller drops. Two suggestions: (1) increase the
proportion of protein in the - technical term - teeny drop to say two
thirds and (2) cover the drops with oil eg Al's oils
(silicone/paraffin). You still get vapor diffusion though the oil ,
and you'd like to slow up equilibration. of course (2) slows up the
robotics a little, but both should be trivial to set up..

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CCP4bb <-- 2008 <-- January 2008 <-- 18 January 2008

Previous message:
Subject: Re: differences between Rsym and Rmerge
From: "Santarsiero, Bernard D {- dot -} " bds {- at -} UIC {- dot -} EDU
Date: 2008-01-18

Next message:
Subject: Re: differences between Rsym and Rmerge
From: William Scott wgscott {- at -} CHEMISTRY {- dot -} UCSC {- dot -} EDU
Date: 2008-01-18