Re: [ccp4bb] Inclusion body

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

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- X-ray detectors

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Highest shell standards
From: Poul Nissen pn {- at -} MB {- dot -} AU {- dot -} DK
Date: 2007-03-24

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Subject: Chi-by-Eye[ Highest shell standards]
From: Bart Hazes Bart {- dot -} Hazes {- at -} UALBERTA {- dot -} CA
Date: 2007-03-24

Subject: Re: Inclusion body
From: Hongmin Li lih {- at -} WADSWORTH {- dot -} ORG
Date: 2007-03-24

Hi Weijun and all,

Anyone tried the Stratagene's ArcticExpress^(TM) Competent Cells for
expression of proteins at low temperature (13 degree). The company
claims that the cells encode chaperonins Cpn60 and Cpn10 and they can
enhance the protein folding and solubility.

Hongmin

>Dear All,

>I got enclusion body in many cases when I tried to express human
>proteins in E coli. I would like some suggestions on how to go about
>it. I would also like to try co-expression of GroEL/GroES or
>DnaJ/DnaK, and would like to know where to get the plasmids.

>Any help or comments would be appreciated.
>Weijun

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Highest shell standards
From: Poul Nissen pn {- at -} MB {- dot -} AU {- dot -} DK
Date: 2007-03-24

Next message:
Subject: Chi-by-Eye[ Highest shell standards]
From: Bart Hazes Bart {- dot -} Hazes {- at -} UALBERTA {- dot -} CA
Date: 2007-03-24