[ccp4bb] Codon Optimized Expression

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: CNS 1.2 on SGI
From: David Garboczi dgarboczi {- at -} NIAID {- dot -} NIH {- dot -} GOV
Date: 2008-02-01

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Subject: Re: Codon Optimized Expression
From: Ray Stevens stevens {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-01

Subject: Codon Optimized Expression
From: "Looney, Andrea Lynn (Andrea Hevrdeys)" hevrdeys {- at -} UTK {- dot -} EDU
Date: 2008-02-01

Dear All,

I am getting very little (not zero) expression of my protein. I am curious to know if it is worthwhile to codon optimize my gene, which is ~1200bp, for E.coli. Can you have "too much of a good thing"? Can codon optimizing overwhelm the cell or in some way cause death or lowered expression?

Thank you all,

Andrea L. Hevrdeys

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: CNS 1.2 on SGI
From: David Garboczi dgarboczi {- at -} NIAID {- dot -} NIH {- dot -} GOV
Date: 2008-02-01

Next message:
Subject: Re: Codon Optimized Expression
From: Ray Stevens stevens {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-01