Re: [ccp4bb] Codon Optimized Expression

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Codon Optimized Expression
From: "Looney, Andrea Lynn (Andrea Hevrdeys)" hevrdeys {- at -} UTK {- dot -} EDU
Date: 2008-02-01

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Subject: Re: Codon Optimized Expression
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-02-01

Subject: Re: Codon Optimized Expression
From: Ray Stevens stevens {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-01

Andrea,

Our experience with whole gene synthesis is that it often does not increase levels of expression (or hurt), but it does allow one to more efficiently make lots of "daughter" constructs from the original "parent" construct. Most companies will charge X for the parent construct depending on the length, and then will provide the daughter constructs at a much lower cost since the DNA is being made anyway for the original. One can also design in restriction sites to allow for more efficient design afterwards. It is likely that by trying several different truncations (N and C termini, large and small), you will increase your chances of success. The above being said, we are occasionally surprised to get increases, so it is always worth a try.

Ray Stevens

________________________________
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Looney, Andrea Lynn (Andrea Hevrdeys)
Sent: Friday, February 01, 2008 7:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Codon Optimized Expression

Dear All,

I am getting very little (not zero) expression of my protein. I am curious to know if it is worthwhile to codon optimize my gene, which is ~1200bp, for E.coli. Can you have "too much of a good thing"? Can codon optimizing overwhelm the cell or in some way cause death or lowered expression?

Thank you all,

Andrea L. Hevrdeys

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Codon Optimized Expression
From: "Looney, Andrea Lynn (Andrea Hevrdeys)" hevrdeys {- at -} UTK {- dot -} EDU
Date: 2008-02-01

Next message:
Subject: Re: Codon Optimized Expression
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-02-01