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Re: [ccp4bb] Codon Optimized Expression
 

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CCP4bb <-- 2008 <-- February 2008 <-- 01 February 2008
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Subject: Re: Codon Optimized Expression
From: Ray Stevens stevens {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-01 		Next message:
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Subject: Re: Codon Optimized Expression
From: Chun Luo chun {- at -} ACCELAGEN {- dot -} COM
Date: 2008-02-01
Andrea,



Check your gene at http://nihserver.mbi.ucla.edu/RACC/ to see whether you
have many rare codon clusters. With many rare codons, you may try those
Codon-plus strains first. The effects of codon optimization is random,
helpful to some genes but not to others. We had a few cases that are even
detrimental to expression.



Best,



Chun



Chun Luo, Ph.D.
The Protein Expert
Accelagen, Inc.
11585 Sorrento Valley Road, Suite 107
San Diego, CA 92121
TeL: 858-350-8085 ext 111
Fax: 858-350-8001
chun@accelagen.com
www.accelagen.com





_____

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Looney, Andrea Lynn (Andrea Hevrdeys)
Sent: Friday, February 01, 2008 7:29 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Codon Optimized Expression



Dear All,



I am getting very little (not zero) expression of my protein. I am curious
to know if it is worthwhile to codon optimize my gene, which is ~1200bp,
for E.coli. Can you have "too much of a good thing"? Can codon optimizing
overwhelm the cell or in some way cause death or lowered expression?



Thank you all,



Andrea L. Hevrdeys
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