- Protein crystallography
Main steps: - Protein purification
- Crystallisation
Special: - Programs for crystallography
- X-ray detectors
Basic tutorials: - Chemistry
- Protein
- Peptide
- Amino Acids
Xtal community: - CCP4BB
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Subject: Re: Codon Optimized Expression
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2008-02-02
One classical way to optimize expression level is to screen culture conditions.
For my proteins, I solved my expression problems by changing the
expression vector to a pET or changing a pET 20 to a pET 30 (if the
protein is toxic).
But keep in mind that a low but folded expression is better than a
high expression to inclusion body.
Michel.
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