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Re: [ccp4bb] Codon Optimized Expression
 

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CCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008
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Subject: Re: Python 3.0 breakage
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
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Subject: Re: Codon Optimized Expression
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-02-02


Subject: Re: Codon Optimized Expression
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2008-02-02
One classical way to optimize expression level is to screen culture conditions.

For my proteins, I solved my expression problems by changing the
expression vector to a pET or changing a pET 20 to a pET 30 (if the
protein is toxic).

But keep in mind that a low but folded expression is better than a
high expression to inclusion body.

Michel.

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008
Previous message:
Subject: Re: Python 3.0 breakage
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-02-02 		Next message:
Subject: Re: Codon Optimized Expression
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-02-02


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