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Re: [ccp4bb] Codon Optimized Expression
 

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CCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008
Previous message:
Subject: Re: Codon Optimized Expression
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2008-02-02 		Next message:
Subject: Re: Codon Optimized Expression
From: Partha Chakrabarti ppchak {- at -} GMAIL {- dot -} COM
Date: 2008-02-02


Subject: Re: Codon Optimized Expression
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-02-02
On Feb 2, 2008, at 2:15 AM, M T wrote:
> One classical way to optimize expression level is to screen culture
> conditions.
>
> For my proteins, I solved my expression problems by changing the
> expression vector to a pET or changing a pET 20 to a pET 30 (if the
> protein is toxic).
>
> But keep in mind that a low but folded expression is better than a
> high expression to inclusion body.

Anecdotal evidence also suggests to try picking several colonies from
the original cloning procedure and doing test expressions with each.
Some clones express better than others even though they should in
principle express identically. I have no idea what the mechanism for
this differential expression could be.

--
James Stroud
UCLA-DOE Institute for Genomics and Proteomics
Box 951570
Los Angeles, CA 90095

http://www.jamesstroud.com

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008
Previous message:
Subject: Re: Codon Optimized Expression
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2008-02-02 		Next message:
Subject: Re: Codon Optimized Expression
From: Partha Chakrabarti ppchak {- at -} GMAIL {- dot -} COM
Date: 2008-02-02


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