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Re: [ccp4bb] Codon Optimized Expression
 

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CCP4bb <-- 2008 <-- February 2008 <-- 02 February 2008
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Subject: Re: Codon Optimized Expression
From: James Stroud jstroud {- at -} MBI {- dot -} UCLA {- dot -} EDU
Date: 2008-02-02 		Next message:
Subject: Re: cannot read .mtz in ccp4i
From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-02


Subject: Re: Codon Optimized Expression
From: Partha Chakrabarti ppchak {- at -} GMAIL {- dot -} COM
Date: 2008-02-02
Hi Andrea,

We have seen examples where it can make a dramatic difference, no
expression even with codon plus strains, but really good expression
with the daughter constructs as already pointed out. It is
particularly true for parasite proteins where the percentage of AT is
higher than bacteria. Also for mammalian kinases etc.

However, it can become a maximisation and not optimisation, hence can
lead to trouble regarding toxicity or solubility. But there are
expression system other than IPTG induced where one can fine tune
these things.

Regards, Partha



On Feb 1, 2008 3:29 PM, Looney, Andrea Lynn (Andrea Hevrdeys)
wrote:
>
>
>
> Dear All,
>
> I am getting very little (not zero) expression of my protein. I am curious
> to know if it is worthwhile to codon optimize my gene, which is ~1200bp,
> for E.coli. Can you have "too much of a good thing"? Can codon optimizing
> overwhelm the cell or in some way cause death or lowered expression?
>
> Thank you all,
>
> Andrea L. Hevrdeys
>



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