Re: [ccp4bb] Codon Optimized Expression

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Codon Optimized Expression
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-02-02

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Subject: Re: Codon Optimized Expression
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-02-02

Subject: Re: Codon Optimized Expression
From: price {- at -} UCHICAGO {- dot -} EDU price {- at -} UCHICAGO {- dot -} EDU
Date: 2008-02-02

Rumor also has it that more than one "bad" codon in a row, particular
near the beginning, can be extra-bad. For one small, A/T rich
protein, we simply "fixed" a pair of bad Arg codons near the
N-terminus at the same time as recloning it, by using the N-terminal
cloning primer to do the mutagenesis. Combined with using codon-plus
E coli, expression improved dramatically.
Good luck!
Phoebe Rice

At 09:29 AM 2/1/2008, Looney, Andrea Lynn (Andrea Hevrdeys) wrote:
>Dear All,
>
>I am getting very little (not zero) expression of my protein. I am
>curious to know if it is worthwhile to codon optimize my gene, which
>is ~1200bp, for E.coli. Can you have "too much of a good
>thing"? Can codon optimizing overwhelm the cell or in some way
>cause death or lowered expression?
>
>Thank you all,
>
>Andrea L. Hevrdeys
>

---------------------------------------------------------------------------------------------------------------------------
Phoebe A. Rice
Assoc. Prof., Dept. of Biochemistry & Molecular Biology
The University of Chicago
phone 773 834 1723
fax 773 702 0439
http://bmb.bsd.uchicago.edu/Faculty_and_Research/01_Faculty/01_Faculty_Alphabetically.php?faculty_id=123
http://www.nasa.gov/mission_pages/cassini/multimedia/pia06064.html

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Codon Optimized Expression
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-02-02

Next message:
Subject: Re: Codon Optimized Expression
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-02-02