Re: [ccp4bb] His tag

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From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
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Date: 2008-02-04

Subject: Re: His tag
From: Bostjan Kobe b {- dot -} kobe {- at -} UQ {- dot -} EDU {- dot -} AU
Date: 2008-02-04

Dear Yanming

Lots of proteins have been crystallized with His-tags on. However, in
general one would assume that a flexible tag could have a negative effect.

I am not aware of a systematic comparison of crystallization of tagged and
untagged proteins, but the following paper is relevant to this topic:

Carson M, Johnson DH, McDonald H, Brouillette C, Delucas LJ.
His-tag impact on structure.
Acta Crystallogr D Biol Crystallogr. 2007 Mar;63(Pt 3):295-301. Epub 2007
Feb 21.
PMID: 17327666 [PubMed - indexed for MEDLINE]

I would expect the same protein with the tags on different ends would often
crystallize under similar conditions, as long as the tags do not interfere
with the packing in the crystal.

Bostjan

On 5/2/08 11:54 AM, "Yanming Zhang" wrote:

> Hi All,
> Maybe, I should not have asked this question:
> Can anybody give me the hints (or point to the references) on the impact
> of His tag on crystallization experiments. In perticular:
> 1, With or without His tag, which one is better for crystallization?
> 2, If I successfully crystallized N-terminal, what information can I get
> to assist my set-up of C-terminal crystallization set-up.
>
> Sorry for taking up your time because of my inadequate knowledge
> Thank you for your help
> Yanming

---
Bostjan Kobe
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Professor of Structural Biology
School of Molecular and Microbial Sciences
and Institute for Molecular Bioscience
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CCP4bb <-- 2008 <-- February 2008 <-- 04 February 2008

Previous message:
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From: Tim Gruene tg {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
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Subject: Re: His tag
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-02-04