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Re: [ccp4bb] extra line of stain in our gel
 

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CCP4bb <-- 2008 <-- February 2008 <-- 07 February 2008
Previous message:
Subject: extra line of stain in our gel
From: Michael Colaneri colanerim {- at -} GMAIL {- dot -} COM
Date: 2008-02-07 		Next message:
Subject: Announcement: two crystallographic wikis
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-02-07


Subject: Re: extra line of stain in our gel
From: fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT fenguita {- at -} ITQB {- dot -} UNL {- dot -} PT
Date: 2008-02-07
Hi Mike

Sometimes keratin from your skin (fingers, nails, etc) appear in the gels
as a band around 48-52 kDa. Take care in the handling of the
electrophoresis should be a solution.

Other possible reason could be a contamination of the sample loading
buffer, also with keratin or with other proteins.

Hope it helps

Cheers

Francisco



> Hello,
>
> When we develop our gel we recently keep getting a horizontal line of
> stain
> at 50000 mol weight in all lanes of our gel. (This is not a feature of
> interest except that the protein that we are interested happens to be
> 50000
> mol weight). I would appreciate any ideas on how we can get rid of this
> line.
>
> Thanks. Mike
>


-----------------------------------------
Francisco J. Enguita, Ph.D.
Host-pathogen Interactions Group
Macromolecular Crystallography Laboratory
ITQB
EAN, Av. da República
2781-901 Oeiras
Portugal
Phone : +351-21-4469663
Fax : +351-21-4433644
E-mail : fenguita@itqb.unl.pt
-----------------------------------------

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 07 February 2008
Previous message:
Subject: extra line of stain in our gel
From: Michael Colaneri colanerim {- at -} GMAIL {- dot -} COM
Date: 2008-02-07 		Next message:
Subject: Announcement: two crystallographic wikis
From: Kay Diederichs Kay {- dot -} Diederichs {- at -} UNI-KONSTANZ {- dot -} DE
Date: 2008-02-07


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