Re: [ccp4bb] extra line of stain in our gel

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CCP4bb <-- 2008 <-- February 2008 <-- 07 February 2008

Previous message:
Subject: Re: an over refined structure
From: Doug Ohlendorf ohlen {- at -} UMN {- dot -} EDU
Date: 2008-02-07

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Subject: Re: Announcement: two crystallographic wikis
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-07

Subject: Re: extra line of stain in our gel
From: Dima Klenchin klenchin {- at -} FACSTAFF {- dot -} WISC {- dot -} EDU
Date: 2008-02-07

>
>When we develop our gel we recently keep getting a horizontal line of
>stain at 50000 mol weight in all lanes of our gel. (This is not a
>feature of interest except that the protein that we are interested happens
>to be 50000 mol weight). I would appreciate any ideas on how we can get
>rid of this line.

Are these somewhat fuzzy bands? If yes then it's [human] keratins. Usually
it is either SDS or bME stocks that get contaminated with keratins.
Usually, just changing stocks and working cleanly solves the issue but
occasionally bME as bought already contains them.

Ochs, D. (1983) Protein contaminants of sodium dodecyl sulfatepolyacrylamide
gels. Anal. Biochem. 135, 470–474.

Dima

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 07 February 2008

Previous message:
Subject: Re: an over refined structure
From: Doug Ohlendorf ohlen {- at -} UMN {- dot -} EDU
Date: 2008-02-07

Next message:
Subject: Re: Announcement: two crystallographic wikis
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-02-07