[ccp4bb] Tag, you're in! Flag, V5, etc

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 07 February 2008

Previous message:
Subject: Re: an over refined structure
From: Edward Berry eaberry {- at -} LBL {- dot -} GOV
Date: 2008-02-07

Next message:
Subject: Re: Tag, you're in! Flag, V5, etc
From: James Whisstock James {- dot -} Whisstock {- at -} MED {- dot -} MONASH {- dot -} EDU {- dot -} AU
Date: 2008-02-07

Subject: Tag, you're in! Flag, V5, etc
From: "Y {- dot -} -F {- dot -} Li" yongfuli {- at -} GMAIL {- dot -} COM
Date: 2008-02-07

Hello,

I'd appreciate it if anyone could provide information (experiences or
publications) on the following:

1. Put a tag such as FLAG, V5, etc etc, at the N- and/or C-termini, in order
for specific detection, but not interfering with protein folding/structure;
2. Is a linker between the tag and target protein needed? What linkers
(length, specific sequences) would you suggest?
3. What are the choices of such tags and why would you recommend them?

Thank you for your time and sharing in advance.

Yongfu Li

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 07 February 2008

Previous message:
Subject: Re: an over refined structure
From: Edward Berry eaberry {- at -} LBL {- dot -} GOV
Date: 2008-02-07

Next message:
Subject: Re: Tag, you're in! Flag, V5, etc
From: James Whisstock James {- dot -} Whisstock {- at -} MED {- dot -} MONASH {- dot -} EDU {- dot -} AU
Date: 2008-02-07