[ccp4bb] Engineering disulfide bonds

Protein crystallography Programs for crystallography X-ray detectors
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Subject: Re: an over refined structure
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-02-08

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Subject: Re: Engineering disulfide bonds
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2008-02-08

Subject: Engineering disulfide bonds
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-08

I'm trying to engineer a disulfide bond into a protein that has several
other cysteines.

My question is whether there is a crystallization friendly reducing agent
that can be used to prevent oxidation of the free cysteines without breaking
the disulfide?

Also, can I expect 100% disulfide formation from standard bacterial
expression (assuming good geometry of the cysteines)?

Thanks,

Kendall
--
Kendall W. Nettles, PhD
Assistant Professor
Department of Cancer Biology
The Scripps Research Institute
5353 Parkside Dr.
Jupiter Fl 33458

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 08 February 2008

Previous message:
Subject: Re: an over refined structure
From: Ian Tickle I {- dot -} Tickle {- at -} ASTEX-THERAPEUTICS {- dot -} COM
Date: 2008-02-08

Next message:
Subject: Re: Engineering disulfide bonds
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2008-02-08