Re: [ccp4bb] Engineering disulfide bonds

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Subject: Engineering disulfide bonds
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-08

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From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
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Subject: Re: Engineering disulfide bonds
From: "David J {- dot -} Schuller" djs63 {- at -} CORNELL {- dot -} EDU
Date: 2008-02-08

On Fri, 2008-02-08 at 10:44 -0500, Kendall Nettles wrote:

> Also, can I expect 100% disulfide formation from standard bacterial
> expression (assuming good geometry of the cysteines)?

No, E. coli cells are a reducing environment.

-
=======================================================================
With the single exception of Cornell, there is not a college in the
United States where truth has ever been a welcome guest - R.G. Ingersoll
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David J. Schuller
modern man in a post-modern world
MacCHESS, Cornell University
schuller@cornell.edu

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CCP4bb <-- 2008 <-- February 2008 <-- 08 February 2008

Previous message:
Subject: Engineering disulfide bonds
From: Kendall Nettles knettles {- at -} SCRIPPS {- dot -} EDU
Date: 2008-02-08

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Subject: Re: Engineering disulfide bonds
From: Cynthia Kinsland clk10 {- at -} CORNELL {- dot -} EDU
Date: 2008-02-08