Re: [ccp4bb] Thermofluor

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From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2008-02-12

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Subject: Re: Thermofluor
From: Derek Logan derek {- dot -} logan {- at -} MBFYS {- dot -} LU {- dot -} SE
Date: 2008-02-12

Subject: Re: Thermofluor
From: Martin Hallberg Martin {- dot -} Hallberg {- at -} KI {- dot -} SE
Date: 2008-02-12

Hi Jeroen,

We used a BioRad iCycler iQ for the measurements in
"Thermoï¬‚uor-based high-throughput stability optimization of proteins
for
structural studies." Ericsson, et. al. Analytical Biochemistry (2006)
357:289-298.
The wavelengths for excitation and detection were ~490 and ~575 nm,
respectively (for Sypro Orange).
See http://probes.invitrogen.com/handbook/figures/0020.html for
wavelength (band) selection.
The iCycler iQ is highly dependable and very sensitive.

Best regards,

Martin

On Feb 12, 2008, at 6:07 PM, mesters wrote:

> Sorry for the off-topic but can somebody recommend highly a
> sensitive RT-PCR machine for the thermofluor experiment (sypro
> orange).
> That would imply excitation below 500 nm (ideally 470) and detection
> at about 570 nm, right? I know several simple machines have a
> problem with the 570 nm...
>
> Jeroen.

.
B. Martin Hallberg, PhD
Assistant professor
Department of Cell and Molecular Biology
Medical Nobel Institute
Karolinska Institutet
von Eulers v. 1
SE-171 77 Stockholm
Sweden

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CCP4bb <-- 2008 <-- February 2008 <-- 12 February 2008

Previous message:
Subject: summary: sgi dials on intel mac
From: Andreas_Förster docandreas {- at -} GMAIL {- dot -} COM
Date: 2008-02-12

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Subject: Re: Thermofluor
From: Derek Logan derek {- dot -} logan {- at -} MBFYS {- dot -} LU {- dot -} SE
Date: 2008-02-12