[ccp4bb] refinement problem of labelled RNA complex

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Subject: refinement problem of labelled RNA complex
From: ºîº£·å houhf {- at -} IHEP {- dot -} AC {- dot -} CN
Date: 2008-02-18

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From: Ronnie Berntsson r {- dot -} berntsson {- at -} RUG {- dot -} NL
Date: 2008-02-18

Subject: refinement problem of labelled RNA complex
From: ºîº£·å houhf {- at -} IHEP {- dot -} AC {- dot -} CN
Date: 2008-02-18

Dear all;
I am a fresher of Refmac5. And now I'm trying to refine a protein and labelled RNA complex using Refmac. It's a Iodo-U labelled RNA. Unfortunately, these is no appropriate lib file in the current refmac dictionaries. So I use cif file which created by Refmc itself, surprisingly, the base of uridine distorted greatly. Could someone like to tell me how to make the appropriate cif file to solve this problem. Another question is that the RNA has a phosphate group in 5', How to define this in the cif file?

Could someone help me? Thanks a lot!
Best wishes,

Hai-Feng Hou

Biological macromolecule group
Beijing Synchrotron Radiation Facility
Institute of High Energy Physics
Chinese Academy of Sciences
Beijing, China
Tel: +86-10-88234208
E-mail: houhf@ihep.ac.cn

2008-02-18

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008

Previous message:
Subject: refinement problem of labelled RNA complex
From: ºîº£·å houhf {- at -} IHEP {- dot -} AC {- dot -} CN
Date: 2008-02-18

Next message:
Subject: Re: crashing-out protein eluted from Nickel column
From: Ronnie Berntsson r {- dot -} berntsson {- at -} RUG {- dot -} NL
Date: 2008-02-18