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Re: [ccp4bb] phasing power ofatoms
 

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CCP4bb <-- 2007 <-- March 2007 <-- 28 March 2007
Previous message:
Subject: EMBO workshop: 'RNA viruses: replication, evolution and drug design' - cosponsored by VIZIER
From: Kristina Djinovic Carugo kristina {- dot -} djinovic {- at -} UNIVIE {- dot -} AC {- dot -} AT
Date: 2007-03-28 		Next message:
Subject: Valid to stop Refmac after TLS refinement?
From: "NM Burton, Biochemistry" Nick {- dot -} Burton {- at -} BRISTOL {- dot -} AC {- dot -} UK
Date: 2007-03-28


Subject: Re: phasing power ofatoms
From: artis {- at -} CHEMISTRY {- dot -} MONTANA {- dot -} EDU artis {- at -} CHEMISTRY {- dot -} MONTANA {- dot -} EDU
Date: 2007-03-28
Hello Gordon,
Theres two that I know of...
Anomalous signal indicators in protein crystallography by P.H. Zwart,
Biological crystallography 2005, D61, 1437-1448

Estimation of anomalous signal in diffraction data, Zbigniew Dauter,
Biological Crystallography, 2006, D62, 867-876.

The Bijvoet ratio gives an idea.
‹
ΔF±
› /‹
F
› =(2NA/NP)1/2 / (f”/f˚eff)
(thats raised to power 1/2)
NA- number of anomalous atoms
NP - number of protein atoms
f˚eff=atomic scattering factor of average protein atom=6.7
f"=atomic scattering factor of anomalous scattering atom at a certain
wavelength.

Arti



Arti S Pandey
Chemistry and Biochemistry
Montana State University
Bozeman MT 59717
Hello everyone,
>
>
>
> I would like to know the phasing power of different heavy atoms ie how
> many seleniums per 10 kDa would be good enough to solve the structure. I
> wonder is there a good website or some useful publications that people
> know of, that would give this information, not just for Selenium but for
> other atoms as well.
>
>
>
> Thanks in advance,
>
>
>
> M. Gordon Joyce,
>
> Visiting Research Fellow,
>
> Structural Immunology Section,
>
> Laboratory of Immunogenetics,
>
> NIH/NIAID,
>
> 12441 Parklawn Drive,
>
> Rockville,
>
> MD 20851
>
>
>
> Phone: 301 594 0242 Office
>
> 301 496 3792 Lab
>
>
>
>

CCP4bb navigation

CCP4bb <-- 2007 <-- March 2007 <-- 28 March 2007
Previous message:
Subject: EMBO workshop: 'RNA viruses: replication, evolution and drug design' - cosponsored by VIZIER
From: Kristina Djinovic Carugo kristina {- dot -} djinovic {- at -} UNIVIE {- dot -} AC {- dot -} AT
Date: 2007-03-28 		Next message:
Subject: Valid to stop Refmac after TLS refinement?
From: "NM Burton, Biochemistry" Nick {- dot -} Burton {- at -} BRISTOL {- dot -} AC {- dot -} UK
Date: 2007-03-28


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