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Re: [ccp4bb] Check the conformation of one important amino acid

 

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CCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008
Previous message:
Subject: Re: PISA parameters for surface area calculation
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-02-18
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Subject: Re: Check the conformation of one important amino acid
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-02-19


Subject: Re: Check the conformation of one important amino acid
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-02-18

Dear Sun --

> I want to check the conformation of one important amino acid in the
> structure by looking the difference density map. Should I just omit
> that amino acid in the refinement or should I also omit its
> flanking amino acids? Which one is better?

The real expert will talk better than me, but I'd go for removing 1-2
additional residues around your target. Personal preferences
though... Depends on your resolution as well. If at low resolution
you remove 3 residues, you might have all of them gone for good, so
the target would be enough...

HTH

Kind regards.

Leo
------------------------------------------------------------
Chavas Leonard, Ph.D.
Research Associate
------------------------------------------------------------
Faculty of Life Sciences
The University of Manchester
The Michael Smith Building
Oxford Road
Manchester Lancashire
M13 9PT
------------------------------------------------------------
Tel: +44(0)161-275-1586
e-mail: Leonard.Chavas@manchester.ac.uk



CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008
Previous message:
Subject: Re: PISA parameters for surface area calculation
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-02-18
Next message:
Subject: Re: Check the conformation of one important amino acid
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2008-02-19



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