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Re: [ccp4bb] Check the conformation of one important amino acid |
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CCP4bb navigationCCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008Subject: Re: Check the conformation of one important amino acid From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM Date: 2008-02-18 > I want to check the conformation of one important amino acid in the > structure by looking the difference density map. Should I just omit > that amino acid in the refinement or should I also omit its > flanking amino acids? Which one is better? The real expert will talk better than me, but I'd go for removing 1-2 additional residues around your target. Personal preferences though... Depends on your resolution as well. If at low resolution you remove 3 residues, you might have all of them gone for good, so the target would be enough... HTH Kind regards. Leo ------------------------------------------------------------ Chavas Leonard, Ph.D. Research Associate ------------------------------------------------------------ Faculty of Life Sciences The University of Manchester The Michael Smith Building Oxford Road Manchester Lancashire M13 9PT ------------------------------------------------------------ Tel: +44(0)161-275-1586 e-mail: Leonard.Chavas@manchester.ac.uk CCP4bb navigationCCP4bb <-- 2008 <-- February 2008 <-- 18 February 2008 |
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