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Re: [ccp4bb] Side chain chemical modifiers
 

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CCP4bb <-- 2008 <-- February 2008 <-- 21 February 2008
Previous message:
Subject: Side chain chemical modifiers
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-02-21 		Next message:
Subject: Re: XDS and overlaps
From: "Coker A {- dot -} R {- dot -} " A {- dot -} Coker {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2008-02-21


Subject: Re: Side chain chemical modifiers
From: "Das, Debanu" debanu {- at -} SLAC {- dot -} STANFORD {- dot -} EDU
Date: 2008-02-21
Hi,
You can use dimethylamine borane (DMAB) for reductive methylation of the surface exposed K's. You may also try NaBH4 or sodium cyanoborohydride but the first may be best.
Don't know about D's and E's.

Best,
Debanu.

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob Keller
Sent: Thursday, February 21, 2008 2:32 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Side chain chemical modifiers

Dear Crystallographers,

I am looking for a way to modify any specific set of sidechains in my protein (e.g., all K's, or all D's and E's, etc.). A few requirements:

1. Relatively cheap
2. Definitely non-cell-membrane-crossing 3. Specific 4. Any reasonable amount of change in mass (+ 5-5000 Da?), charge, etc., is fine 5. Preferably less life threatening to live cells (I know this is a probably a losing battle)

The last criterion is because I want to modify the protein in vivo, then immediately harvest the cells, etc. I was hoping there would be something "around the house" that I could use for this, especially for hitting K's.

All the best,

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

CCP4bb navigation

CCP4bb <-- 2008 <-- February 2008 <-- 21 February 2008
Previous message:
Subject: Side chain chemical modifiers
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-02-21 		Next message:
Subject: Re: XDS and overlaps
From: "Coker A {- dot -} R {- dot -} " A {- dot -} Coker {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2008-02-21


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