Re: [ccp4bb] Side chain chemical modifiers

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From: "Coker A {- dot -} R {- dot -} " A {- dot -} Coker {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2008-02-21

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Subject: Re: Side chain chemical modifiers
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-02-21

Most reagents that modify side chains are also (not surprisingly) toxic.
Many of them are small and therefore membrane-permeable.

Look up recent works on in vivo 'click chemistry'. Unfortunately I doubt
that these compounds can be classified as 'cheap'.

If you want to do this in vivo, you're probably better off using biology
rather than chemistry - i.e. enzyme tagging. If you are able to disclose
more details on what are you trying to accomplish perhaps this forum may be
of better use :)

Artem

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of Jacob
Keller
Sent: Thursday, February 21, 2008 5:32 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Side chain chemical modifiers

Dear Crystallographers,

I am looking for a way to modify any specific set of sidechains in my
protein (e.g., all K's, or all D's and E's, etc.). A few requirements:

1. Relatively cheap
2. Definitely non-cell-membrane-crossing
3. Specific
4. Any reasonable amount of change in mass (+ 5-5000 Da?), charge, etc., is
fine
5. Preferably less life threatening to live cells (I know this is a probably

a losing battle)

The last criterion is because I want to modify the protein in vivo, then
immediately harvest the cells, etc. I was hoping there would be something
"around the house" that I could use for this, especially for hitting K's.

All the best,

Jacob

*******************************************
Jacob Pearson Keller
Northwestern University
Medical Scientist Training Program
Dallos Laboratory
F. Searle 1-240
2240 Campus Drive
Evanston IL 60208
lab: 847.491.2438
cel: 773.608.9185
email: j-keller2@northwestern.edu
*******************************************

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CCP4bb <-- 2008 <-- February 2008 <-- 21 February 2008

Previous message:
Subject: Re: XDS and overlaps
From: "Coker A {- dot -} R {- dot -} " A {- dot -} Coker {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2008-02-21

Next message:
Subject: Re: cns and refmac refinement
From: Chavas Leo ccp4hnaalas {- at -} GMAIL {- dot -} COM
Date: 2008-02-21