[ccp4bb] Multiple nucleation

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Commensurate superlattice discussion; summing up and thankyou
From: John R Helliwell jrhelliwell {- at -} GMAIL {- dot -} COM
Date: 2007-03-29

Next message:
Subject: Re: Multiple nucleation
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-29

Subject: Multiple nucleation
From: Jobichen Chacko jobichenc {- at -} NUS {- dot -} EDU {- dot -} SG
Date: 2007-03-29

Hai All,
Sorry for the non Ccp4 question.
I got very small crystals for a protein and I am trying to optimize the condition to improve the size of the crystal and reduce the number of nucleation.
The crystals are coming from five to six conditions , all have PEG 3350 in common.
Apart from PEG, the condition has Lithium sulphate and Bis Tris in one condition and Ammonium suphate and HEPES in another condition, while the third condition is Sodium malonate, Bis Tris and PEG. I am getting thousands of small crystals with some precipitation.
I tried macroseeding and additive screen, but the crystals are not growing.
Any suggestions are more than welcome.
Thank you.
Jobi

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Commensurate superlattice discussion; summing up and thankyou
From: John R Helliwell jrhelliwell {- at -} GMAIL {- dot -} COM
Date: 2007-03-29

Next message:
Subject: Re: Multiple nucleation
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-29