Re: [ccp4bb] Multiple nucleation

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Multiple nucleation
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-03-30

Next message:
Subject: Re: Multiple nucleation
From: "Radisky, Evette S {- dot -} Ph {- dot -} D {- dot -} " Radisky {- dot -} Evette {- at -} MAYO {- dot -} EDU
Date: 2007-03-30

Subject: Re: Multiple nucleation
From: Mario Sanches msanches {- at -} LNLS {- dot -} BR
Date: 2007-03-30

You said you tried macroseeding, but what about microseeding? It works
quite well in many cases. Just don't forget that you should find a zone
were nucleation does not happen before you seed. Take a look at this paper:

Bergfors, T. (2003) Seeds to Crystals, J. Struct. Biol., 142, 66-76.

There is also other methods to separate nucleation and growth. Take a
look at

Saridakis, E., and Chayen, N.E. (2000) Improving protein crystal quality
by decoupling nucleation and growth in vapor diffusion, Protein Sci., 9,
755-757

If you have access to a DLS equipment you may try

Saridakis, E., Dierks, K., Moreno, A., Dieckmann, W.M., and Chayen, N.E.
(2002) Separating nucleation and growth in protein crystallization using
dynamic light scattering, Acta Cryst. Sect. D, 58, 1597-1600.

HTH,

Mario Sanches

Jobichen Chacko wrote:

> Hai All,
> Sorry for the non Ccp4 question.
> I got very small crystals for a protein and I am trying to optimize
> the condition to improve the size of the crystal and reduce the number
> of nucleation.
> The crystals are coming from five to six conditions , all have PEG
> 3350 in common.
> Apart from PEG, the condition has Lithium sulphate and Bis Tris in one
> condition and Ammonium suphate and HEPES in another condition, while
> the third condition is Sodium malonate, Bis Tris and PEG. I am
> getting thousands of small crystals with some precipitation.
> I tried macroseeding and additive screen, but the crystals are not
> growing.
> Any suggestions are more than welcome.
> Thank you.
> Jobi
>
>
>
>

--
Mario Sanches
Laboratório Nacional de Luz Síncrotron
Cristalografia e Espectroscopia de Biomoléculas
Tel: +55 19 3512 1109

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Multiple nucleation
From: Frank von Delft frank {- dot -} vondelft {- at -} SGC {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2007-03-30

Next message:
Subject: Re: Multiple nucleation
From: "Radisky, Evette S {- dot -} Ph {- dot -} D {- dot -} " Radisky {- dot -} Evette {- at -} MAYO {- dot -} EDU
Date: 2007-03-30