| Quick navigation: | Home | Site Map || References | Biography || Copyright | Other copyright | Contact us | Advert | | |
Re: [ccp4bb] Multiple nucleation |
||
- Protein crystallographyMain steps:- Protein purification- Crystallisation Special:- Programs for crystallography- X-ray detectors Basic tutorials:- Chemistry- Protein - Peptide - Amino Acids Xtal community:- CCP4BB |
CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999Subject: Re: Multiple nucleation From: Mario Sanches msanches {- at -} LNLS {- dot -} BR Date: 2007-03-30 You said you tried macroseeding, but what about microseeding? It works quite well in many cases. Just don't forget that you should find a zone were nucleation does not happen before you seed. Take a look at this paper: Bergfors, T. (2003) Seeds to Crystals, J. Struct. Biol., 142, 66-76. There is also other methods to separate nucleation and growth. Take a look at Saridakis, E., and Chayen, N.E. (2000) Improving protein crystal quality by decoupling nucleation and growth in vapor diffusion, Protein Sci., 9, 755-757 If you have access to a DLS equipment you may try Saridakis, E., Dierks, K., Moreno, A., Dieckmann, W.M., and Chayen, N.E. (2002) Separating nucleation and growth in protein crystallization using dynamic light scattering, Acta Cryst. Sect. D, 58, 1597-1600. HTH, Mario Sanches Jobichen Chacko wrote: > Hai All, > Sorry for the non Ccp4 question. > I got very small crystals for a protein and I am trying to optimize > the condition to improve the size of the crystal and reduce the number > of nucleation. > The crystals are coming from five to six conditions , all have PEG > 3350 in common. > Apart from PEG, the condition has Lithium sulphate and Bis Tris in one > condition and Ammonium suphate and HEPES in another condition, while > the third condition is Sodium malonate, Bis Tris and PEG. I am > getting thousands of small crystals with some precipitation. > I tried macroseeding and additive screen, but the crystals are not > growing. > Any suggestions are more than welcome. > Thank you. > Jobi > > > > -- Mario Sanches Laboratório Nacional de Luz Síncrotron Cristalografia e Espectroscopia de Biomoléculas Tel: +55 19 3512 1109 CCP4bb navigationCCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999 |
|
| ProteinCrystallography.org: Copyright 2006-2010 by Quid United Ltd |