[ccp4bb] Crosslinking reagents

Protein crystallography Programs for crystallography X-ray detectors
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CCP4bb <-- 2008 <-- March 2008 <-- 02 March 2008

Previous message:
Subject: Re: anomalous signal of Mn and Ca ions
From: Andrzej Olczak andrzej {- at -} MBSKRYS {- dot -} P {- dot -} LODZ {- dot -} PL
Date: 2008-03-01

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Subject: Correct the dihedral angles
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2008-03-02

Subject: Crosslinking reagents
From: Nian Huang huangnian {- at -} GMAIL {- dot -} COM
Date: 2008-03-02

Dear all,
I know most people use glutaraldehyde as the crosslinking reagent for
their crystals. But there are a lot of other crosslinking chemicals
available both in protein chemistry and histology, such as simple
formaldehyde. Did anybody have experience with other chemicals and how
did they compared with glutaradehyde? I have a crystal very sensitive
to glutaradehyde but I really wants to try the crosslinking method.
Thanks,

Nian Huang
Dept of Biochemistry
UT Southwestern Medical Center
Dallas, TX 75390

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 02 March 2008

Previous message:
Subject: Re: anomalous signal of Mn and Ca ions
From: Andrzej Olczak andrzej {- at -} MBSKRYS {- dot -} P {- dot -} LODZ {- dot -} PL
Date: 2008-03-01

Next message:
Subject: Correct the dihedral angles
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2008-03-02