Re: [ccp4bb] Multiple nucleation

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Multiple nucleation
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-30

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Subject: Job Opportunity for Programmer
From: "Leslie A {- dot -} " andrew {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-03-30

Subject: Re: Multiple nucleation
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2007-03-30

I've found that crystallization in sitting drops under oil dramatically reduces the no. of nucleation events and increases the overall crystal size. Now, if the increased crystal size helps improve diffraction is something to be tested.

Among the many other suggestions...
Raji

---------Included Message----------
>Hai All,
>Sorry for the non Ccp4 question.
>I got very small crystals for a protein and I am trying to optimize the condition to improve the size of the crystal and reduce the number of nucleation.
>The crystals are coming from five to six conditions , all have PEG 3350 in common.
>Apart from PEG, the condition has Lithium sulphate and Bis Tris in one condition and Ammonium suphate and HEPES in another condition, while the third condition is Sodium malonate, Bis Tris and PEG. I am getting thousands of small crystals with some
precipitation.
>I tried macroseeding and additive screen, but the crystals are not growing.
>Any suggestions are more than welcome.
>Thank you.
>Jobi
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---------End of Included Message----------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Multiple nucleation
From: artem {- at -} XTALS {- dot -} ORG artem {- at -} XTALS {- dot -} ORG
Date: 2007-03-30

Next message:
Subject: Job Opportunity for Programmer
From: "Leslie A {- dot -} " andrew {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2007-03-30