Re: [ccp4bb] finicky protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: new versions of Mosflm (7.0.3) and iMosflm (0.6.1)
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-03-03

Next message:
Subject: finicky protein
From: Mads Gabrielsen mg118m {- at -} UDCF {- dot -} GLA {- dot -} AC {- dot -} UK
Date: 2008-03-03

Subject: Re: finicky protein
From: David M Shechner shechner {- at -} MIT {- dot -} EDU
Date: 2008-03-03

Quoting James Stroud :

> Try refolding before purification.

OR, you could do a denaturing purification, followed by refolding. Ni-NTA's
perfectly viable in denaturing urea, for example. From there, it's just a
question of whether to try refolding on-resin, or following elution.

Cheers,
d.s.

===========================================
David M. Shechner, Graduate Student
Bartel Laboratory
Department of Biology
Whitehead Institute for Biomedical Research
Massachusetts Institute of Technology
shechner@mit.edu
===========================================

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: new versions of Mosflm (7.0.3) and iMosflm (0.6.1)
From: Harry Powell harry {- at -} MRC-LMB {- dot -} CAM {- dot -} AC {- dot -} UK
Date: 2008-03-03

Next message:
Subject: finicky protein
From: Mads Gabrielsen mg118m {- at -} UDCF {- dot -} GLA {- dot -} AC {- dot -} UK
Date: 2008-03-03