[ccp4bb] protein degraded when setting xtal?

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: finicky protein / cell disruption
From: Thomas Earnest TNEarnest {- at -} LBL {- dot -} GOV
Date: 2008-03-03

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Date: 2008-03-03

Subject: protein degraded when setting xtal?
From: Ngo Duc Tri ngoductri {- at -} GMAIL {- dot -} COM
Date: 2008-03-03

Dear CCP4 experts,
I'm working on a kind of cystein peptidase. It shows a little degradation
after 2 days storing at 4 degree even I tried many kinds of protease
inhibitors, or glycerol and DTT.
So I decided to work fast (1 day) then set xtal. After one week I pick up
some condition and check degradation by SDS-PAGE. It shows heavy degradation
then I think it's impossible to obtain the crystal if I don't find out how
to stop the degradation first.

Do you have any idea about my situation? Is it true that I need to stop the
degradation and how to deal with it?
Thank you for your advices,

My best regards,
TriNgo

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: finicky protein / cell disruption
From: Thomas Earnest TNEarnest {- at -} LBL {- dot -} GOV
Date: 2008-03-03

Next message:
Subject: 2008 Rigaku Post-doc Summer Travel Bursary Awards
From: Jim Pflugrath jim {- dot -} pflugrath {- at -} RIGAKU {- dot -} COM
Date: 2008-03-03