Re: [ccp4bb] Crosslinking reagents

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Positions available in Australia
From: Margaret Bills margaret {- dot -} bills {- at -} MED {- dot -} MONASH {- dot -} EDU {- dot -} AU
Date: 2008-03-03

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Subject: HIC-Update: Release 12.1 @ 2008-03-03 ==> 7,870 compounds
From: Gerard DVD Kleywegt gerard {- at -} XRAY {- dot -} BMC {- dot -} UU {- dot -} SE
Date: 2008-03-03

Subject: Re: Crosslinking reagents
From: Nian Huang huangnian {- at -} GMAIL {- dot -} COM
Date: 2008-03-03

I used glutaraldehyde vapor by placing crystal drop above a bridge
with 15% glutaradehyde for various amount of time. It worked for me
for many crystals, but not for the one that I am trying now. The
crystal didn't diffract any more. I intended to make the crystal
tougher for heavy metal soaking. I am just wondering what will happen
if I use some other crosslinking chemicals.

Nian Huang

Dept of Biochemistry
UT Southwestern Medical Center
Dallas, TX 75390

On Sun, Mar 2, 2008 at 12:28 AM, Nian Huang wrote:
> Dear all,
> I know most people use glutaraldehyde as the crosslinking reagent for
> their crystals. But there are a lot of other crosslinking chemicals
> available both in protein chemistry and histology, such as simple
> formaldehyde. Did anybody have experience with other chemicals and how
> did they compared with glutaradehyde? I have a crystal very sensitive
> to glutaradehyde but I really wants to try the crosslinking method.
> Thanks,
>
> Nian Huang
> Dept of Biochemistry
> UT Southwestern Medical Center
> Dallas, TX 75390
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Positions available in Australia
From: Margaret Bills margaret {- dot -} bills {- at -} MED {- dot -} MONASH {- dot -} EDU {- dot -} AU
Date: 2008-03-03

Next message:
Subject: HIC-Update: Release 12.1 @ 2008-03-03 ==> 7,870 compounds
From: Gerard DVD Kleywegt gerard {- at -} XRAY {- dot -} BMC {- dot -} UU {- dot -} SE
Date: 2008-03-03