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Re: [ccp4bb] Crosslinking reagents
 

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CCP4bb <-- 2008 <-- March 2008 <-- 03 March 2008
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Date: 2008-03-03


Subject: Re: Crosslinking reagents
From: Nian Huang huangnian {- at -} GMAIL {- dot -} COM
Date: 2008-03-03
I used glutaraldehyde vapor by placing crystal drop above a bridge
with 15% glutaradehyde for various amount of time. It worked for me
for many crystals, but not for the one that I am trying now. The
crystal didn't diffract any more. I intended to make the crystal
tougher for heavy metal soaking. I am just wondering what will happen
if I use some other crosslinking chemicals.

Nian Huang

Dept of Biochemistry
UT Southwestern Medical Center
Dallas, TX 75390

On Sun, Mar 2, 2008 at 12:28 AM, Nian Huang wrote:
> Dear all,
> I know most people use glutaraldehyde as the crosslinking reagent for
> their crystals. But there are a lot of other crosslinking chemicals
> available both in protein chemistry and histology, such as simple
> formaldehyde. Did anybody have experience with other chemicals and how
> did they compared with glutaradehyde? I have a crystal very sensitive
> to glutaradehyde but I really wants to try the crosslinking method.
> Thanks,
>
> Nian Huang
> Dept of Biochemistry
> UT Southwestern Medical Center
> Dallas, TX 75390
>
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