Re: [ccp4bb] Copper Staining

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Subject: Copper Staining
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-03-03

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From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-03

Subject: Re: Copper Staining
From: Rebecca Phillips rephilli {- at -} CHEM {- dot -} UCSD {- dot -} EDU
Date: 2008-03-03

One down side is that copper sulfate is toxic to fish and plants. Please
be good to the environment and do not pour copper sulfate solutions down
the drain. We normally precipitate with NaOH, filter, and put the
precipitated copper in an appropriate waste.

-----Original Message-----
From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jacob Keller
Sent: Tuesday, March 04, 2008 12:03 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Copper Staining

Dear Crystallographers:

just to share something I found out recently, that is really helpful to
know:

5 min Copper Stain protocol

Normal SDS-PAGE gels can be stained in 5 min with 300mM CuCl2. Simply
remove
the gel, rinse ~10s in water, place 5 min in CuCl2, and the gel is
negatively stained, with protein bands appearing clear, and background
opaque white. To visualize, I put it on a flatbed scanner in a darkened
room. It works in my hands every bit as well as coomassie (at least as
sensitive, if not more), and if you want, you can stain afterwards in
coomassie with the usual effect. To destain, place gel in EDTA. The
original
paper is Lee et al, Anal. Biochem. 1987. Instead of waiting hours, you can
see the results in 5 min with copper! Also, there is no smell, and it can
be
re-used, to a point. Gels are stable in H2O for months, say Lee et al.

Jacob

p.s. I am not in copper comodities...
p.p.s. if anybody knows of down-sides to this, please let me know.

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CCP4bb <-- 2008 <-- March 2008 <-- 03 March 2008

Previous message:
Subject: Copper Staining
From: Jacob Keller j-keller2 {- at -} MD {- dot -} NORTHWESTERN {- dot -} EDU
Date: 2008-03-03

Next message:
Subject: Re: Copper Staining
From: Artem Evdokimov artem {- at -} XTALS {- dot -} ORG
Date: 2008-03-03