Re: [ccp4bb] Right Handedness of Density

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: ccp4mg on FC6
From: Dritan Siliqi dritan {- dot -} siliqi {- at -} IC {- dot -} CNR {- dot -} IT
Date: 2007-03-30

Next message:
Subject: Re: Right Handedness of Density
From: "Green, Todd" green {- at -} CBSE {- dot -} UAB {- dot -} EDU
Date: 2007-03-30

Subject: Re: Right Handedness of Density
From: Ruchi Anand ranand {- at -} WISTAR {- dot -} ORG
Date: 2007-03-30

Dear All,
We are working with a DNA binding protein with 4 Zn sites in in ASU. Using SnB and then CNS we were able to get a map using MAD phasing and could visualize the density for the double helix of the DNA but it was a left handed spiral instead of the usual right handed one. The space group which yielded the result was P3(2). We tried to flip the sites and change the space group to P3(1) but we are not able to generate a sensible map. Any advice regarding the best way to proceed will be very helpful. The resolution is around 3.2.
Thanks
Ruchi

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: ccp4mg on FC6
From: Dritan Siliqi dritan {- dot -} siliqi {- at -} IC {- dot -} CNR {- dot -} IT
Date: 2007-03-30

Next message:
Subject: Re: Right Handedness of Density
From: "Green, Todd" green {- at -} CBSE {- dot -} UAB {- dot -} EDU
Date: 2007-03-30