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Re: [ccp4bb] Right Handedness of Density |
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CCP4bb navigationCCP4bb <-- 2007 <-- March 2007 <-- 30 March 2007Subject: Re: Right Handedness of Density From: Ruchi Anand ranand {- at -} WISTAR {- dot -} ORG Date: 2007-03-30 We are working with a DNA binding protein with 4 Zn sites in in ASU. Using SnB and then CNS we were able to get a map using MAD phasing and could visualize the density for the double helix of the DNA but it was a left handed spiral instead of the usual right handed one. The space group which yielded the result was P3(2). We tried to flip the sites and change the space group to P3(1) but we are not able to generate a sensible map. Any advice regarding the best way to proceed will be very helpful. The resolution is around 3.2. Thanks Ruchi CCP4bb navigationCCP4bb <-- 2007 <-- March 2007 <-- 30 March 2007 |
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