[ccp4bb] protein expression

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: phenix.refine and refmac
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2008-03-04

Next message:
Subject: Re: phenix.refine and refmac
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2008-03-04

Subject: protein expression
From: Daniel Jin jin_xiechen {- at -} YAHOO {- dot -} COM
Date: 2008-03-04

Hi,

I have a protein with two independently folded domains. I can express either one in bacteria with pretty good expression yield. However, when I put them together with a linker, the expression drops significantly. I can barely see any soluble protein and most of it is now inclusion bodies. I used the same bacteria strain and expression/purification conditions. Is it normal? Any suggestion about how to improve? Many thanks.

Best,
Chen

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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: phenix.refine and refmac
From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM
Date: 2008-03-04

Next message:
Subject: Re: phenix.refine and refmac
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2008-03-04