Re: [ccp4bb] protein expression

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: phenix.refine and refmac
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2008-03-04

Next message:
Subject: Problem with DM
From: yuhua yuhua {- dot -} lan {- at -} GMAIL {- dot -} COM
Date: 2008-03-04

Subject: Re: protein expression
From: wu donghui wdh0972 {- at -} GMAIL {- dot -} COM
Date: 2008-03-04

Hi Chen,

In this case, it seems that linker region is of great importance for the
proper folding of the two linked domains. I have not much experience in
linker region design, generally use (GS)5-10 times. However it depends on
individual case. Anyone who has successful experience in linker region
design might share your success with others. Thanks a lot.

Regards,

Donghui

On 3/5/08, Daniel Jin wrote:
>
> Hi,
>
> I have a protein with two independently folded domains. I can express
> either one in bacteria with pretty good expression yield. However, when I
> put them together with a linker, the expression drops significantly. I can
> barely see any soluble protein and most of it is now inclusion bodies. I
> used the same bacteria strain and expression/purification conditions. Is it
> normal? Any suggestion about how to improve? Many thanks.
>
> Best,
> Chen
>
>
>
> ------------------------------
> Be a better friend, newshound, and know-it-all with Yahoo! Mobile. Try it
> now.
>
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: phenix.refine and refmac
From: Pavel Afonine PAfonine {- at -} LBL {- dot -} GOV
Date: 2008-03-04

Next message:
Subject: Problem with DM
From: yuhua yuhua {- dot -} lan {- at -} GMAIL {- dot -} COM
Date: 2008-03-04