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[ccp4bb] Problem with DM

 

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CCP4bb <-- 2008 <-- March 2008 <-- 04 March 2008
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Subject: Re: protein expression
From: wu donghui wdh0972 {- at -} GMAIL {- dot -} COM
Date: 2008-03-04
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Subject: Re: Removal of glycosylation sites in Picha expression construct
From: hua jing jing_hua {- at -} HOTMAIL {- dot -} COM
Date: 2008-03-05


Subject: Problem with DM
From: yuhua yuhua {- dot -} lan {- at -} GMAIL {- dot -} COM
Date: 2008-03-04

Dear Crystallographers,

I failed to run DM in ccp4 package recently and got the errors as below:

dm
reference:


K. Cowtan (1994),
dm: An automated procedure for phase improvement by density modification.
Joint CCP4 and ESF-EACBM Newsletter on Protein Crystallography, 31,
p34-38.




Contents




Command Input



Data line--- MODE
SOLV
Data line--- COMBINE
PERT
Data line--- SCHEME
ALL
Data line--- NCYCLE
AUTO
Data line--- SOLCONT


*** Warning
Real sub-argument expected

Data line--- NCSMASK

Data line--- LABIN
FP=F_p2 SIGFP=SIGF_p2 PHIO=PHIC FOMO=FOM
Data line--- LABOUT
FDM=FDM PHIDM=PHIDM FOMDM=FOMDM
dm: Input error (see above)
Times: User: 0.0s System: 0.0s Elapsed: 0:00


***************************************************************************
* Information from CCP4Interface script
***************************************************************************
The program run with command: dm HKLIN
"/Users/Research/CNS/newtls_refmac4.mtz" HKLOUT
"/Users/Research/CNS/newtls_dm1.mtz" SOLOUT
"/Users/Research/CNS/newtls_dm1.msk"
has failed with error message
dm: Input error (see above)
***************************************************************************


#CCP4I TERMINATION STATUS 0 " dm: Input error (see above)"
#CCP4I TERMINATION TIME 04 Mar 2008 22:15:09
#CCP4I MESSAGE Task failed


I can not fix it. Can anyone give me some help?
Thanks very much!

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 04 March 2008
Previous message:
Subject: Re: protein expression
From: wu donghui wdh0972 {- at -} GMAIL {- dot -} COM
Date: 2008-03-04
Next message:
Subject: Re: Removal of glycosylation sites in Picha expression construct
From: hua jing jing_hua {- at -} HOTMAIL {- dot -} COM
Date: 2008-03-05



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