[ccp4bb] isopropanol as a precipitant

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: Removal of glycosylation sites in Picha expression construct
From: hua jing jing_hua {- at -} HOTMAIL {- dot -} COM
Date: 2008-03-05

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Subject: Re: isopropanol as a precipitant
From: Tommi Kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2008-03-05

Subject: isopropanol as a precipitant
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2008-03-05

Dear all
Sorry for non-CCP4 query.
I have crystallized a 7kDa protein in 50-60% of isopropanol,pH
4.0-4.6.Theinteresting thing is that the xtal appears within 5 hrs at
16 degree.
The protein conc is 5mg/ml and drop size is 3+1 with mother liquor 300 micro
lt.Numerous xtals appears and but small is size.Do anyone can share their
experience with Isopropanol as a precipitant and to improve the xtal quality
with other precipitants.All suggestions are welcome.
Thanx in advance.
Shivesh

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Removal of glycosylation sites in Picha expression construct
From: hua jing jing_hua {- at -} HOTMAIL {- dot -} COM
Date: 2008-03-05

Next message:
Subject: Re: isopropanol as a precipitant
From: Tommi Kajander tommi {- dot -} kajander {- at -} HELSINKI {- dot -} FI
Date: 2008-03-05