Re: [ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec

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CCP4bb <-- 2007 <-- March 2007 <-- 31 March 2007

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Subject: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: jjwarren {- at -} DUKE {- dot -} EDU jjwarren {- at -} DUKE {- dot -} EDU
Date: 2007-03-31

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Subject: Refmac refinement problem
From: Todd Holyoak tholyoak {- at -} KUMC {- dot -} EDU
Date: 2007-03-31

Subject: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: tea {- dot -} pavkov {- at -} UNI-GRAZ {- dot -} AT tea {- dot -} pavkov {- at -} UNI-GRAZ {- dot -} AT
Date: 2007-03-31

did you think about to do (or send your samples) for the edman degradation?

-----Original Message-----
From: CCP4 bulletin board on behalf of Yong Tang
Sent: Sat 3/31/2007 7:05 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec

Dear all, just a super dummy question: I treated a protein with
trypsin, found the protein being degraded into two well-define
fragments, ran a sizing column to find them co-elute, sent the peak
fraction for mass-spec, got the two masses. Now here is the question -
is there any program readily available for me to roughly identify
these two (around 20K) fragments with the full-length sequence and
these two masses, and of cause, with the fact that I use trypsin to
cut it? I checked a lot of programs listed on Expasy to find them
mostly dealing with smaller peptide length. Any information would be
highly appreciated. Thanks and have a nice weekend, -yong

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CCP4bb <-- 2007 <-- March 2007 <-- 31 March 2007

Next message:
Subject: Refmac refinement problem
From: Todd Holyoak tholyoak {- at -} KUMC {- dot -} EDU
Date: 2007-03-31