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Re: [ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec |
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CCP4bb navigationCCP4bb <-- 2007 <-- March 2007 <-- 31 March 2007Subject: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec From: tea {- dot -} pavkov {- at -} UNI-GRAZ {- dot -} AT tea {- dot -} pavkov {- at -} UNI-GRAZ {- dot -} AT Date: 2007-03-31 -----Original Message----- From: CCP4 bulletin board on behalf of Yong Tang Sent: Sat 3/31/2007 7:05 AM To: CCP4BB@JISCMAIL.AC.UK Subject: [ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec Dear all, just a super dummy question: I treated a protein with trypsin, found the protein being degraded into two well-define fragments, ran a sizing column to find them co-elute, sent the peak fraction for mass-spec, got the two masses. Now here is the question - is there any program readily available for me to roughly identify these two (around 20K) fragments with the full-length sequence and these two masses, and of cause, with the fact that I use trypsin to cut it? I checked a lot of programs listed on Expasy to find them mostly dealing with smaller peptide length. Any information would be highly appreciated. Thanks and have a nice weekend, -yong CCP4bb navigationCCP4bb <-- 2007 <-- March 2007 <-- 31 March 2007 |
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