Re: [ccp4bb] Molecular replacement of a multidomain protein

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: MTZ cell troubles after sortmtz reindexing from P321 to C2
From: "George M {- dot -} Sheldrick" gsheldr {- at -} SHELX {- dot -} UNI-AC {- dot -} GWDG {- dot -} DE
Date: 2008-03-07

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Subject: Re: Molecular replacement of a multidomain protein
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-03-07

Subject: Re: Molecular replacement of a multidomain protein
From: Lucas Bleicher lbleicher {- at -} YAHOO {- dot -} COM
Date: 2008-03-07

I've had a very good experience with MrBump:

http://www.ccp4.ac.uk/MrBUMP/

Not only because of the program itself, which was able
to find an unexpected template for the problematic
chain (the first one was straightforward in Phaser),
but also because of great support from Martyn & Ron.
It's definitely worth a try.

Lucas

--- Anjali Mehta escreveu:

> Dear All,
> I am working with a Bifunctional protein of
> molecular weight ~60 kDa.
> I have a 3.3 angstrom native dataset. The matthews
> number show there are 6
> molecules in the asymmetric unit.
> The structures of the individual domains are already
> known from prokaryotes.
> The sequence identity with the known structures are
> about 30%.
> I have tried molecular replacement using the two
> parts as models
> respectively with CNS, MOLREP, PHASER etc. However I
> always get the solution
> for one domain. I have also tried to fix that domain
> and find the other one.
> But none of the programs can find a solution.
> I am trying to model build the correct sequence of
> one domain using a
> density modified (using CNS), NCS averaged (using
> RAVE) map but the map does
> not look very good. The side chains are not clear.
> That might be due to the
> fact that I am only having a partial model.
> Any suggestion will be appreciated.
> Thanks.
> Ms. Anjali Mehta
>

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: Re: Molecular replacement of a multidomain protein
From: Roger Rowlett rrowlett {- at -} MAIL {- dot -} COLGATE {- dot -} EDU
Date: 2008-03-07