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Re: [ccp4bb] (bigger) fragment identification of limited proteolysis w/ mass-spec
 

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CCP4bb <-- 2007 <-- March 2007 <-- 31 March 2007
Previous message:
Subject: Re: Refmac refinement problem
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-31 		Next message:
Subject: Fwd: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: Paul Kraft haresear68 {- at -} YAHOO {- dot -} COM
Date: 2007-04-01


Subject: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: Juergen Bosch jbosch {- at -} U {- dot -} WASHINGTON {- dot -} EDU
Date: 2007-03-31
You should be able to solve your problem with the following link:

http://us.expasy.org/tools/findpept.html

Jürgen

Yong Tang wrote:

> Dear all, just a super dummy question: I treated a protein with
> trypsin, found the protein being degraded into two well-define
> fragments, ran a sizing column to find them co-elute, sent the peak
> fraction for mass-spec, got the two masses. Now here is the question -
> is there any program readily available for me to roughly identify
> these two (around 20K) fragments with the full-length sequence and
> these two masses, and of cause, with the fact that I use trypsin to
> cut it? I checked a lot of programs listed on Expasy to find them
> mostly dealing with smaller peptide length. Any information would be
> highly appreciated. Thanks and have a nice weekend, -yong
>


--
Jürgen Bosch
University of Washington
Dept. of Biochemistry, K-426
1705 NE Pacific Street
Seattle, WA 98195
Box 357742
Phone: +1-206-616-4510
FAX: +1-206-685-7002

CCP4bb navigation

CCP4bb <-- 2007 <-- March 2007 <-- 31 March 2007
Previous message:
Subject: Re: Refmac refinement problem
From: Garib Murshudov garib {- at -} YSBL {- dot -} YORK {- dot -} AC {- dot -} UK
Date: 2007-03-31 		Next message:
Subject: Fwd: Re: (bigger) fragment identification of limited proteolysis w/ mass-spec
From: Paul Kraft haresear68 {- at -} YAHOO {- dot -} COM
Date: 2007-04-01


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