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Re: [ccp4bb] process SeMet labelled data

 

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CCP4bb <-- 2007 <-- March 2007 <-- 01 March 2007
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Subject: Re: Oxford Xcalibur Vs Rigaku micromax
From: sankar {- at -} MKUSTRBIOINFO {- dot -} COM
Date: 2007-03-01
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Subject: Re: Cannot running NTA to purify the protein having His-tag?
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Date: 2007-03-01


Subject: Re: process SeMet labelled data
From: dirk {- dot -} kostrewa {- at -} PSI {- dot -} CH
Date: 2007-03-01

Hi Mark,

although Shivesh's question was not very specific, and he should have
clearly given some more informations about what he would like to know,
he is probably a beginner in crystallography and simply asked for help
on this board. Not everyone has always time or is always in the mood to
answer such questions. In my opinion, it's then better not to respond at
all than to give an answer like yours that is neither helpful nor funny!
We all should try to keep a good style here.

Dirk.

Mark J. van Raaij wrote:
> why don't you just send all your images to the ccp4bb, then we'll
> process them, solve the structure and publish it for you.
> And we might put you in the acknowledgements, if you are lucky.
> Mark
> On 28 Feb 2007, at 16:35, Jonathan Grimes wrote:
>
>> Anastassis Perrakis wrote:
>>> On Feb 28, 2007, at 14:37, shivesh kumar wrote:
>>>
>>>> Dear all,
>>>> I have a data set at 2.2A, of the selenomethionene labelled
>>>> protein.How should I process the data.
>>>
>>> Carefully !
>>>
>>>> Thanx for the help.
>>>> Shivesh
>>>
>>> Tassos
>>
>>
>> i am sure what tassos really meant was "Very Carefully !"
>>
>> jon
>>
>> --
>> Dr. Jonathan M. Grimes, Royal Society Research Fellow University
>> Research Lecturer
>> Division of Structural Biology
>> Wellcome Trust Centre for Human Genetics
>> University of Oxford
>> Roosevelt Drive,
>> Oxford OX3 7BN, UK
>>
>> Email: Jonathan@strubi.ox.ac.uk, Web: www.strubi.ox.ac.uk Tel: (+44) -
>> 1865 - 287561, FAX: (+44) - 1865 - 287547
>
> Mark J. van Raaij
> Dpto de Bioquímica, Facultad de Farmacia
> and
> Unidad de Rayos X, Edificio CACTUS
> Universidad de Santiago
> 15782 Santiago de Compostela
> Spain
> http://web.usc.es/~vanraaij/
>
>
>


--

****************************************
Dirk Kostrewa
Paul Scherrer Institut
Biomolecular Research, OFLC/110
CH-5232 Villigen PSI, Switzerland
Phone: +41-56-310-4722
Fax: +41-56-310-5288
E-mail: dirk.kostrewa@psi.ch
http://sb.web.psi.ch
****************************************

CCP4bb navigation

CCP4bb <-- 2007 <-- March 2007 <-- 01 March 2007
Previous message:
Subject: Re: Oxford Xcalibur Vs Rigaku micromax
From: sankar {- at -} MKUSTRBIOINFO {- dot -} COM
Date: 2007-03-01
Next message:
Subject: Re: Cannot running NTA to purify the protein having His-tag?
From: joost {- dot -} uitdehaag {- at -} ORGANON {- dot -} COM
Date: 2007-03-01



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