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Re: [ccp4bb] Color of heme containing Xtals
 

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CCP4bb <-- 2008 <-- March 2008 <-- 12 March 2008
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Subject: Re: Color of heme containing Xtals
From: Ian Ollmann iano {- at -} APPLE {- dot -} COM
Date: 2008-03-12 		Next message:
Subject: Re: Color of heme containing Xtals
From: R Michael Garavito garavito {- at -} MSU {- dot -} EDU
Date: 2008-03-12


Subject: Re: Color of heme containing Xtals
From: "Sanishvili, Ruslan" rsanishvili {- at -} ANL {- dot -} GOV
Date: 2008-03-12
Crystals are supposed to be red if you have oxidized iron in the heme
and deep pink/scarlet if the iron is reduced.
If you removed the iron (for example EDTA during purification) or
substituted with something during purification or crystallization, you
could loose the color.
It is trivial to check your crystals on the synchrotron beamline with
fluorescence spectrum and "see" which metals are there.
Cheers,
Nukri


Ruslan Sanishvili (Nukri), Ph.D.

GM/CA-CAT, Bld. 436, D007
Biosciences Division, ANL
9700 S. Cass Ave.
Argonne, IL 60439

Tel: (630)252-0665
Fax: (630)252-0667
rsanishvili@anl.gov





________________________________

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
Jan Schoepe
Sent: Wednesday, March 12, 2008 2:49 PM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] Color of heme containing Xtals


Hello everybody,

I wonder if anybody has experience with heme (or to be more precise:
heme b) containing proteins which Xtals do not look red under the
microscope. How might the technique for crystallization (e.g. sitting
drop, hanging drop) influence the intensity of the color? Many thanks!

Jan


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