Re: [ccp4bb] Tricks to solubilize protein

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Position in protein crystallography, Novartis. Insts. for Biomedical Research - Emeryville
From: "Bussiere, Dirksen" dirksen {- dot -} bussiere {- at -} NOVARTIS {- dot -} COM
Date: 2008-03-16

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Subject: question about processing data
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-03-17

Subject: Re: Tricks to solubilize protein
From: M T michelthe {- at -} GMAIL {- dot -} COM
Date: 2008-03-17

Hi

The low pH and its interaction with PIP1 can be conflicting. The low
pH can modify the interaction site.
Nevertheless, one good way to study protein stability is thermal shift assay.
You can read this publication too : PubMed ID 16604423. (Rapid
determination of protein solubility and stability conditions for NMR
studies using incomplete factorial design).

Michel.

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Next message:
Subject: question about processing data
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-03-17