Quick navigation:        Home   |    Site Map   ||    References   |    Biography   ||    Copyright   |    Other copyright   |    Contact us   |   
Protein structure
 

Re: [ccp4bb] cryoprotectant for crystals in isopropanol
 

Basic tutorials: 		 
 

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 19 March 2008
Previous message:
Subject: Crystal scoring sheets for iPhones/Pocket-PCs
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-03-19 		Next message:
Subject: Senior Scientist Position at Evotec
From: "Mather, Owen" Owen {- dot -} Mather {- at -} EVOTEC {- dot -} COM
Date: 2008-03-19


Subject: Re: cryoprotectant for crystals in isopropanol
From: "Radisky, Evette S {- dot -} , Ph {- dot -} D {- dot -} " Radisky {- dot -} Evette {- at -} MAYO {- dot -} EDU
Date: 2008-03-19
Another anecdote for you: I had crystals that grew in 15% PEG 2000 and
15% isopropanol. Cryos with glycerol melted the crystals. The best
cryo I found was with 15% PEG 2000, 15% isopropanol, and 20% PEG 400.
It's hard to predict how your crystals will behave with different
cryoprotectants-- hopefully you have enough crystals to try several
different conditions.


Evette S. Radisky, Ph.D.
Assistant Professor and Associate Consultant II
Mayo Clinic Cancer Center
Griffin Cancer Research Building, Rm 310
4500 San Pablo Road
Jacksonville, FL 32224
(904) 953-6372 (office)
(904) 953-0046 (lab)



________________________________

From: CCP4 bulletin board [mailto:CCP4BB@JISCMAIL.AC.UK] On Behalf Of
shivesh kumar
Sent: Wednesday, March 19, 2008 3:34 AM
To: CCP4BB@JISCMAIL.AC.UK
Subject: [ccp4bb] cryoprotectant for crystals in isopropanol


Dear all,
The question is regarding the cryoprotectant.We have crystallized a
protein in 20% PEG 500 with 10% isopropanol.What should be the cryo we
should try for data collection.Also,what percentage of PEG 500 is
sufficient enough as a cryo for data collection.
We are also trying to crystallize it with higher PEG's also like 1K, 2K,
4K.Lets C.Since the condition is having isopropanol also and it is
reported that mounting with isoprop is very difficult and it evaporates
as soon as we open the slides.
Thanx in advance...
Shivesh Kumar

CCP4bb navigation

CCP4bb <-- 2008 <-- March 2008 <-- 19 March 2008
Previous message:
Subject: Crystal scoring sheets for iPhones/Pocket-PCs
From: P Hubbard dienoylcoa {- at -} HOTMAIL {- dot -} COM
Date: 2008-03-19 		Next message:
Subject: Senior Scientist Position at Evotec
From: "Mather, Owen" Owen {- dot -} Mather {- at -} EVOTEC {- dot -} COM
Date: 2008-03-19


ProteinCrystallography.org: Copyright 2006-2008 by Quid United Ltd