Re: [ccp4bb] expression vector

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: expression vector
From: Miles Pufall mpufall {- at -} CMP {- dot -} UCSF {- dot -} EDU
Date: 2008-03-27

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From: Lawrence Shapiro lss8 {- at -} COLUMBIA {- dot -} EDU
Date: 2008-03-27

Subject: Re: expression vector
From: junfeng liu jliu {- at -} NIMR {- dot -} MRC {- dot -} AC {- dot -} UK
Date: 2008-03-27

Hi,Yang,
Tag(type and length) ,surface charge and crystallization condition are
parameters which affect the packing in the crystal. It should be the tag
or mutation not the the vector itself made you get different unit cell.
ta
liu
ÑîÁøÇà wrote:
> Hello,everyone!
> I have a question,is the expression vector effect crystallization much?
> For example,"PQE2-protein" structure has 2 molecules in one
> unit-cell,"PQE70-protein single mutant" has 5molecules in one
> unit-cell.Is the vector effect the results or the mutant itself?
> Can protein with some kind vector does not produce crystals?
> Thank you very much!
> Best wishes!
> liuqing
>
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CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: expression vector
From: Miles Pufall mpufall {- at -} CMP {- dot -} UCSF {- dot -} EDU
Date: 2008-03-27

Next message:
Subject: Placing an EM map in a large P1 box
From: Lawrence Shapiro lss8 {- at -} COLUMBIA {- dot -} EDU
Date: 2008-03-27