- Protein crystallography
Main steps: - Protein purification - Crystallisation
Special: - Programs for crystallography - X-ray detectors
Basic tutorials: - Chemistry - Protein - Peptide - Amino Acids
Xtal community: - CCP4BB
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Subject: Question about cryoprotectant From: yang li robertcatrukie {- at -} GMAIL {- dot -} COM Date: 2007-04-03
Hi, l have a crystal grow at condition screen l 40: 0.1M tri-sodium citrate dihydrate pH5.6 isoproponal 20%PEG4k 20% and the crystal need a cryoprotectant, we have used the 30% glycerol but it is not good, the mosaicity of the diffraction pattern is a little high, so anyone knows which is the best cryoprotectant for this crystal? Thanks!
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