[ccp4bb] "inclusion body"

Protein crystallography Programs for crystallography X-ray detectors
Basic tutorials:
Protein Peptide

CCP4bb navigation

CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008

Previous message:
Subject: Re: libstdc++.so.5
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: a question about CNS composite omit map
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-04-02

Subject: "inclusion body"
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2008-04-02

Dear all,
Sorry for the off-topic question...
What can be done to avoid a protein going inside inclusion body.The gene is
cloned in pET30a with C-ter his tag and expressed in BL21-DE3 from 37 to
18C for 3-4 hr with .5mM of IPTG,it is going to inclusion body.All
suggestions are welcome.
Thanx in advance.
Shivesh

CCP4bb navigation

CCP4bb <-- 2008 <-- April 2008 <-- 02 April 2008

Previous message:
Subject: Re: libstdc++.so.5
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: a question about CNS composite omit map
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-04-02