[ccp4bb] "inclusion body"

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

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Subject: Re: libstdc++.so.5
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-04-02

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From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-04-02

Subject: "inclusion body"
From: shivesh kumar 2shivesh {- at -} GMAIL {- dot -} COM
Date: 2008-04-02

Dear all,
Sorry for the off-topic question...
What can be done to avoid a protein going inside inclusion body.The gene is
cloned in pET30a with C-ter his tag and expressed in BL21-DE3 from 37 to
18C for 3-4 hr with .5mM of IPTG,it is going to inclusion body.All
suggestions are welcome.
Thanx in advance.
Shivesh

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: libstdc++.so.5
From: "Winter, G (Graeme)" g {- dot -} winter {- at -} DL {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: a question about CNS composite omit map
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-04-02