Re: [ccp4bb] "inclusion body"

- Protein crystallography

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: a question about CNS composite omit map
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-04-02

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Subject: Inverse 3D profile/threading
From: Jason Greenwald jason {- dot -} greenwald {- at -} PHYS {- dot -} CHEM {- dot -} ETHZ {- dot -} CH
Date: 2008-04-02

Subject: Re: "inclusion body"
From: Brenda Patterson ucbccka {- at -} UCL {- dot -} AC {- dot -} UK
Date: 2008-04-02

Lower temperature, use chaperones (e.g. TAKARA set), refolding?

Quoting shivesh kumar <2shivesh@GMAIL.COM>:

> Dear all,
> Sorry for the off-topic question...
> What can be done to avoid a protein going inside inclusion body.The gene is
> cloned in pET30a with C-ter his tag and expressed in BL21-DE3 from 37 to
> 18C for 3-4 hr with .5mM of IPTG,it is going to inclusion body.All
> suggestions are welcome.
> Thanx in advance.
> Shivesh
>

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: a question about CNS composite omit map
From: Melody Lin xtalin2007 {- at -} GMAIL {- dot -} COM
Date: 2008-04-02

Next message:
Subject: Inverse 3D profile/threading
From: Jason Greenwald jason {- dot -} greenwald {- at -} PHYS {- dot -} CHEM {- dot -} ETHZ {- dot -} CH
Date: 2008-04-02