Re: [ccp4bb] "inclusion body"

- Protein crystallography

Main steps:

- Protein purification
- Crystallisation

Special:

- Programs for crystallography
- X-ray detectors

Basic tutorials:

   - Chemistry
   - Protein
   - Peptide
   - Amino Acids

Xtal community:

- CCP4BB

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Inverse 3D profile/threading
From: Isaac Westwood isaac {- dot -} westwood {- at -} PHARM {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: Re: "inclusion body"
From: Giles Robertson g {- dot -} robertson {- at -} MAIL {- dot -} CRYST {- dot -} BBK {- dot -} AC {- dot -} UK
Date: 2008-04-02

Subject: Re: "inclusion body"
From: Mohinder Pal mp11 {- at -} SOTON {- dot -} AC {- dot -} UK
Date: 2008-04-02

I had the similar problems with the protein expression but I tried heat
shock at 42 degree C for 25 mins before induction with IPTG and
approximately 60% of the overexpressed protein become soluble. The
explanation for this is the upregulation of heat shock proteins that may
help in the refolding of the overexpressed proteins. So it is woth to try.

Best of luck.

Mohinder

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: Inverse 3D profile/threading
From: Isaac Westwood isaac {- dot -} westwood {- at -} PHARM {- dot -} OX {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: Re: "inclusion body"
From: Giles Robertson g {- dot -} robertson {- at -} MAIL {- dot -} CRYST {- dot -} BBK {- dot -} AC {- dot -} UK
Date: 2008-04-02