Re: [ccp4bb] Co-expression plasmids

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CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: "inclusion body"
From: Louise Major llm6 {- at -} ST-ANDREWS {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: twinned?
From: Qiang Chen chen {- at -} RED {- dot -} DFCI {- dot -} HARVARD {- dot -} EDU
Date: 2008-04-02

Subject: Re: Co-expression plasmids
From: Raji Edayathumangalam redayath {- at -} MAIL {- dot -} ROCKEFELLER {- dot -} EDU
Date: 2008-04-02

I do use the Duet vectors extensively and they work just fine. I have trouble with my protein
complex, but that is solely related to my proteins. My lab mate has used these vectors very
successfully.

I have been using pRSF-Duet and pETDuet1 more than pCDFDuet1 or pACYCDuet1 for no specific reason
except for a slight preference for the antibiotics. I avoid pACYCDuet1 (Cam resistance) since a lot
of expression cells I use have plasmids with Cam resistance.

Also, I recently heard about the chaperone co-expression from Takara and folks claim that this
system works well.

Also, some folks recommend pCOLD vectors (Takara). Might be worth looking into if you are shopping
anyway. I don't think these are for co-expression but it is just as easy to stitch in a T7 promoter
and RBS to incorporate additional genes.

Hope that helps.
Raji

---------Included Message----------
>Date: 2-apr-2008 09:56:09 -0400
>From: "Mark J. van Raaij"
>To:
>Subject: [ccp4bb] Co-expression plasmids
>
>Dear All,
>
>Anyone have experience with the NovaGen Duet co-expression vectors? Or
>can recommend others?
>http://www.emdbiosciences.com/html/NVG/Duet_Spot.html
>
>Greetings,
>
>Mark
>
>Mark J. van Raaij
>Dpto de Bioquímica, Facultad de Farmacia
>Universidad de Santiago
>15782 Santiago de Compostela
>Spain
>http://web.usc.es/~vanraaij/
>
>
>
>
>
>
---------End of Included Message----------

CCP4bb navigation

CCP4bb <-- 1999 <-- November 1999 <-- 30 November 1999

Previous message:
Subject: Re: "inclusion body"
From: Louise Major llm6 {- at -} ST-ANDREWS {- dot -} AC {- dot -} UK
Date: 2008-04-02

Next message:
Subject: twinned?
From: Qiang Chen chen {- at -} RED {- dot -} DFCI {- dot -} HARVARD {- dot -} EDU
Date: 2008-04-02